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Erythropoiesis

Erythropoiesis

Red blood cells (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia, and...

Erythropoiesis

Erythropoiesis

Red blood cells (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia, and...

Lifecycle of Erythrocytes

Lifecycle of Erythrocytes

Erythrocytes, also known as red blood cells, constantly move through blood capillaries. As a result, they damage their plasma membrane due to the continuous friction. Typically, after 100 to 120 days, erythrocytes become rigid and fragile as they wear out. As they pass through small vessels in the spleen and liver, they can get trapped and break apart into fragments.
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups.

The Early Endosome: Endocytosis of Transferrin

The Early Endosome: Endocytosis of Transferrin

Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...

Factors Affecting Erythropoiesis

Factors Affecting Erythropoiesis

The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...

Microbes and Other Elemental Cycles

Microbes and Other Elemental Cycles

Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...

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Related Experiment Video

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Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes

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The labile iron pool in human erythroid cells.

Eugenia Prus¹, Eitan Fibach

¹Department of Haematology, Hadassah-Hebrew University Medical Centre, Jerusalem, Israel.

British Journal of Haematology

|May 28, 2008

Summary

The labile iron pool (LIP) decreases during red blood cell maturation. Beta-thalassemia patients show elevated LIP in erythroid cells, suggesting this measurement

Area of Science:

Cellular iron metabolism
Hematology
Biochemistry

Background:

Most cellular iron is bound, but the labile iron pool (LIP) acts as a central hub for iron traffic.
Understanding LIP dynamics is crucial for cellular iron regulation.

Purpose of the Study:

To investigate the labile iron pool (LIP) in human erythroid cells during maturation and in beta-thalassemia.
To assess the utility of flow cytometry for LIP measurement.

Main Methods:

Utilized multi-parameter flow cytometry with calcein and deferiprone to analyze LIP.
Studied LIP in peripheral blood, bone marrow, and cultured erythroid cells.
Investigated LIP changes in normal and beta-thalassemia conditions.

Main Results:

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LIP significantly decreases with erythroid cell maturation.
Nucleated erythrocytes and early reticulocytes exhibit higher LIP than mature cells.
Beta-thalassemia patients show elevated LIP in erythroid cells compared to controls.
Holo-transferrin addition increases LIP in iron-depleted cultures.

Conclusions:

Erythroid cell LIP is modulated by physiological maturation and pathological conditions like beta-thalassemia.
The developed flow cytometry method is valuable for assessing LIP in diseases and evaluating iron chelators.