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Related Experiment Video

Updated: Jul 5, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Thrombin generation before and after multicomponent blood collection.

Christina Cimenti¹, Sabine Sipurzynski, Siegfried Gallistl

¹Department of Pediatrics, Medical University of Graz, Graz, Austria.

|May 28, 2008

Summary

This summary is machine-generated.

Automated blood collection via apheresis does not significantly alter thrombin generation (TG) in donors. This study found no severe changes in the hemostatic system after multicomponent blood collection using cell separators.

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Published on: January 9, 2026

Area of Science:

Hematology
Blood Transfusion Technology
Hemostasis Research

Background:

Apheresis technology advances automated blood collection, improving donor blood use efficiency.
Concerns exist regarding donor blood contact with artificial surfaces and hemostatic system activation.
Thrombin generation (TG) is a key indicator of overall plasmatic clotting system function.

Purpose of the Study:

To evaluate the impact of apheresis on continuous thrombin generation (TG).
To assess potential alterations in the hemostatic system due to automated blood component collection.
To compare TG changes between two different apheresis systems.

Main Methods:

Twenty-six voluntary blood donors underwent apheresis using two cell separators (Amicus and Trima Accel).
Blood samples were collected pre-apheresis, immediately post-apheresis, and 48 hours after.
Continuous thrombin generation (TG) was measured using calibrated automated thrombography (CAT).

Main Results:

Calibrated automated thrombography (CAT) data showed only slight, non-significant changes in TG variables.
No significant differences in TG were observed before, immediately after, or 48 hours post-apheresis (p > 0.05).
TG variables did not differ significantly between the two apheresis systems used (p > 0.05).

Conclusions:

Multicomponent blood collection via apheresis did not induce significant alterations in continuous thrombin generation.
The findings suggest that current apheresis procedures do not lead to severe disruptions in donor hemostatic function.
Calibrated automated thrombography (CAT) provides a sensitive method for assessing hemostatic changes post-apheresis.