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Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope--part II: Multiple

G I Hauser1, S Seiffert, W Oppermann

  • 1Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Strasse 4, D-38678 Clausthal-Zellerfeld, Germany.

Journal of Microscopy
|May 28, 2008
PubMed
Summary

This study introduces a new method for analyzing fluorescence recovery after photo-bleaching (FRAP) data to determine diffusion coefficient distributions. The technique offers a simple, calibration-free approach to quantify multiple diffusion processes observed in microscopy experiments.

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