Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The value of sequential organ failure assessment and its dynamic changes in predicting mortality in hematology intensive care unit].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

Observation of the ϒ(3S) Meson and Suppression of ϒ States in Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2024
Same author

Measurement of the top quark mass using a profile likelihood approach with the lepton + jets final states in proton-proton collisions at <math></math>.

The European physical journal. C, Particles and fields·2023
Same author

Observation of τ Lepton Pair Production in Ultraperipheral Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2023
Same author

Search for Exotic Higgs Boson Decays H→AA→4γ with Events Containing Two Merged Diphotons in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2023
Same author

Observation of Same-Sign WW Production from Double Parton Scattering in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2023

Related Experiment Video

Updated: Jul 5, 2026

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

Nanoparticle shape and configuration analysis by transmission electron tomography.

S P Ahrenkiel1, P R Yu, J E Murphy

  • 1National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, CO 80401, USA. phil.Ahrenkiel@sdsmt.edu

Journal of Microscopy
|May 28, 2008
PubMed
Summary

Transmission electron microscopy tomography reveals nanoparticle shapes and arrangements in 3D. This technique analyzes nanomaterials, showing packing configurations and relating particle shape to crystal structure.

More Related Videos

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
09:19

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
09:19

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

Area of Science:

  • Materials Science
  • Nanotechnology
  • Electron Microscopy

Background:

  • Understanding the 3D structure of nanomaterials is crucial for their properties.
  • Transmission electron microscopy (TEM) is a powerful tool for nanoscale imaging.

Purpose of the Study:

  • To demonstrate the feasibility of transmission electron microscopy (TEM) tomography for 3D nanomaterial analysis.
  • To determine nanoparticle shapes and stacking configurations in ensembles.

Main Methods:

  • Tomographic reconstruction from bright-field image tilt series (up to +/- 70 degrees).
  • Utilized single or dual tilt axes for data acquisition.
  • Employed phase-contrast lattice imaging for crystal structure correlation.

Main Results:

  • Revealed cubic and hexagonal close-packing in indium nanosphere arrays.
  • Correlated the 3D shape of lead selenide (PbSe) octahedral nanoparticles with crystal structure.
  • Confirmed simple-cubic packing in PbSe nanocube multi-layers and observed cubic symmetry in particle shapes.
  • Determined TiO(2) nanorod bundle shapes to be flattened ellipsoids.

Conclusions:

  • TEM tomography is a viable technique for characterizing 3D nanomaterial structures.
  • The study provides detailed insights into nanoparticle packing and shape-crystal structure relationships.
  • Demonstrated the versatility of tomography across different nanomaterials (In, PbSe, TiO(2)).