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Related Concept Videos

Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...
Membrane Fluidity01:26

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Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
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Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

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Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...

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Related Experiment Video

Updated: Jul 5, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

Impact of freezing on high-density lipoprotein functionality.

J Rajinda Kekulawala1, Andrew Murphy, Wilissa D'Souza

  • 1Baker Heart Research Institute, Melbourne, VIC 8008, Australia.

Analytical Biochemistry
|May 28, 2008
PubMed
Summary
This summary is machine-generated.

Short-term freezing preserves high-density lipoprotein (HDL) structure and function, especially with sucrose. Sucrose addition maintains HDL

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Published on: January 28, 2021

Area of Science:

  • Biochemistry
  • Lipid Metabolism
  • Cryopreservation

Background:

  • High-density lipoprotein (HDL) is crucial for reverse cholesterol transport.
  • Maintaining HDL integrity during storage is vital for research and clinical applications.
  • Cryopreservation methods can impact lipoprotein structure and function.

Purpose of the Study:

  • To evaluate the effects of short-term freezing on HDL structural and functional properties.
  • To compare freezing in the presence and absence of sucrose with standard 4°C storage.
  • To determine optimal conditions for preserving HDL integrity during freezing.

Main Methods:

  • Comparison of HDL stored at 4°C versus HDL frozen with or without 10% sucrose.
  • Assessment of HDL particle size.
  • Evaluation of anti-inflammatory and antioxidant properties.
  • Measurement of cholesterol efflux capacity from macrophages.

Main Results:

  • Freezing did not alter HDL particle size, anti-inflammatory, or antioxidant properties.
  • Cholesterol efflux support was slightly impaired by freezing but preserved with sucrose.
  • Approximately 10% loss of HDL was observed in frozen samples.

Conclusions:

  • Freezing HDL in the presence of 10% sucrose effectively preserves its structural and functional integrity.
  • Sucrose addition mitigates the negative impact of freezing on HDL's cholesterol efflux capacity.
  • Cryopreservation with sucrose is a viable method for short-term HDL storage.