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Related Experiment Videos

Transmembrane currents in frog olfactory cilia.

S J Kleene1, R C Gesteland

  • 1Department of Anatomy and Cell Biology, University of Cincinnati, Ohio 45267-0521.

The Journal of Membrane Biology
|February 1, 1991
PubMed
Summary

Researchers measured electrical currents in single frog olfactory cilia. They found that odorant stimulation caused transient currents and that cilia have surprisingly low resistance, suggesting a unique electrical property.

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Area of Science:

  • Olfactory neuroscience
  • Cellular electrophysiology
  • Sensory receptor biology

Background:

  • Olfactory receptor neurons (ORNs) possess a single cilium crucial for odor detection.
  • Understanding the electrical properties of these cilia is vital for comprehending olfactory signal transduction.

Purpose of the Study:

  • To measure transmembrane currents in intact single cilia of frog ORNs.
  • To investigate the electrical excitability and ion channel activity within olfactory cilia.

Main Methods:

  • Utilized patch-clamp electrophysiology on intact single cilia isolated from frog ORNs.
  • Formed high-resistance seals at the cilium base to record ciliary membrane currents.
  • Stimulated cilia with odorants, suction, and voltage ramps.

Main Results:

  • Successfully induced action potentials in single cilia via electrical stimulation.
  • Observed transient currents in response to odorant stimulation.
  • Demonstrated cytoplasmic accessibility of excised cilia to cAMP, indicating functional ion channels.

Conclusions:

  • Single olfactory cilia exhibit measurable electrical activity and excitability.
  • Odorant stimulation can evoke transient currents in olfactory cilia.
  • Olfactory cilia possess a surprisingly low electrical resistance, suggesting unique ion transport mechanisms.

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