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Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
UV–Vis Spectrum01:30

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...

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UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
05:16

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

A new reference material for UV-visible circular dichroism spectroscopy.

Angeliki Damianoglou1, Edward J Crust, Matthew R Hicks

  • 1Department of Chemistry, University of Warwick, Coventry, United Kingdom.

Chirality
|May 29, 2008
PubMed
Summary
This summary is machine-generated.

Accurate circular dichroism (CD) measurements require reliable calibration standards. A new, stable reference material, Na[Co(EDDS)].H(2)O, offers multiple spectral peaks and enantiomeric forms for improved CD instrument calibration and interlaboratory comparability.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Accurate circular dichroism (CD) measurements are crucial for reliable scientific data.
  • Existing CD calibration standards, such as camphor sulfonic acid and ammonium camphor sulfonate, have limitations including poor stability, hygroscopicity, and limited spectral peaks.
  • These limitations hinder consistent and accurate CD instrument calibration across different laboratories and over time.

Purpose of the Study:

  • To synthesize and characterize a novel reference material for calibrating circular dichroism (CD) instruments.
  • To address the shortcomings of current CD calibration standards by providing a stable, versatile material with multiple spectral features.
  • To demonstrate the utility of the new reference material in improving interlaboratory comparability of CD measurements.

Main Methods:

  • Synthesis of the novel CD reference material, Na[Co(EDDS)].H(2)O, in both enantiomeric forms.
  • Characterization of the material's stability and spectral properties, including its CD spectrum with nine distinct peaks between 180 and 599 nm.
  • An interlaboratory comparison study was conducted using the new reference material to assess CD measurement comparability.
  • Development of a fitting algorithm to evaluate CD spectropolarimeter performance.

Main Results:

  • The synthesized Na[Co(EDDS)].H(2)O is an extremely stable reference material available in both enantiomeric forms.
  • Its CD spectrum exhibits nine distinct peaks across a broad spectral range (180-599 nm), offering superior calibration potential.
  • The interlaboratory comparison demonstrated that the new material can enhance the comparability of CD data between different facilities.
  • The developed fitting algorithm provides a method for assessing spectropolarimeter performance.

Conclusions:

  • Na[Co(EDDS)].H(2)O is a highly stable and versatile reference material that overcomes the limitations of existing standards for CD instrument calibration.
  • This new standard has the potential to significantly improve the accuracy, consistency, and comparability of CD measurements globally.
  • The developed fitting algorithm offers a pathway towards a formal quality control process for CD spectropolarimeters.