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Updated: Jul 4, 2026

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
11:30

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Published on: April 21, 2023

Diversity of citrus tristeza virus strains indicated by hybridization with cloned cDNA sequences

A Rosner1, M Bar-Joseph

  • 1Virus Laboratory, Agricultural Research Oganization, The Volcani Center, Bet Dagan, Israel.

Virology
|November 1, 1984
PubMed

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...