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Updated: Jul 4, 2026
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Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
Published on: March 3, 2020
A facile microfluidic method for production of liposomes
Prashant Pradhan1, Jingjiao Guan, Dongning Lu
1Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Anticancer Research
|May 30, 2008
Summary
Researchers developed a microfluidic device for liposome preparation, identifying key factors like needle diameter and lipid concentration that control liposome particle size for tunable production.
Area of Science:
- Biotechnology
- Materials Science
- Nanotechnology
Background:
- Ethanol injection is a common method for liposome preparation.
- Parameters influencing liposome particle size distribution remain incompletely defined.
Purpose of the Study:
- To investigate the parameters affecting liposome particle size distribution using a microfluidic device.
- To establish a method for tunable liposome production.
Main Methods:
- Utilized a syringe pump-driven microfluidic injection device for liposome synthesis.
- Varied conditions such as needle diameter, hydrodynamic pressure, lipid concentration, and membrane rigidity.
Main Results:
- Decreased liposome particle size was achieved by reducing needle diameter or increasing hydrodynamic pressure.
- Smaller liposomes were obtained with lower lipid concentrations and decreased membrane rigidity (lower T(m) or absence of cholesterol).
Conclusions:
- The microfluidic device offers a simple and adaptable method for liposome production.
- This approach enables affordable and tunable control over liposome particle size.
