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Updated: Jul 4, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Published on: June 12, 2017
Mitochondrial AIF protein involved in skeletal muscle regeneration
Xiao-Zhong Qiu1, Lei Yu, Gui-Hua Lai
1Department of Anatomy, Southern Medical University, Guangdong Provinical Tissue Construction and Inspection Key Lab, Guangzhou, Guangdong, China. qqiuxzh@yahoo.com.cn
Abstract:
The mitochondrial flavoprotein apoptosis-inducing factor (AIF) has proved to be either the main mediator of apoptosis or an anti-apoptotic factor via its putative oxidoreductase and peroxide scavenging activities. We report here that 100 muM hydrogen peroxide (H2O2) induced the proliferation of C2C12 myoblasts and over-expression of AIF simultaneously in vitro. Immunofluorescence showed that the over-expression of AIF was located in the cytoplasm. The immunopositive AIF was detected in nuclei 27 days after denervation of skeletal muscle, but in the cytoplasm it was detected 27 days after fiber-damaged skeletal muscle. AIF may be a factor involved in skeletal muscle regeneration.
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