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Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

8.7K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Related Experiment Video

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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain
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Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

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Subcellular proteomics in neuroscience.

Ka Wan Li1, August B Smit

  • 1Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, VU University Amsterdam, The Netherlands. ka.wan.li@cncr.vu.nl

Frontiers in Bioscience : a Journal and Virtual Library
|May 30, 2008
PubMed
Summary
This summary is machine-generated.

Neuroproteomics uses mass spectrometry to study brain proteins and their interactions. This review covers methods for preparing brain samples and performing quantitative neuroproteomics for better understanding brain function and disorders.

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Last Updated: Jan 26, 2026

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Area of Science:

  • Neuroscience
  • Proteomics
  • Genomics

Background:

  • The brain's complexity necessitates advanced molecular investigation.
  • Genomics studies have limitations in revealing underlying molecular mechanisms of brain function and disorders.
  • Quantitative proteomics offers insights into protein interactions and organelle function.

Purpose of the Study:

  • To review common methods for preparing brain subcellular fractions for proteomics.
  • To highlight various approaches for quantitative neuroproteomics.
  • To address the evolving nature and lack of standardized protocols in neuroproteomics technology.

Main Methods:

  • Subcellular fractionation of brain tissue.
  • Mass spectrometry-based quantitative proteomics.
  • Analysis of protein trafficking and interactions.

Main Results:

  • Established methods for preparing brain samples for proteomic analysis.
  • Overview of diverse quantitative neuroproteomics techniques.
  • Identification of key techniques for studying organelle proteome (dys)function.

Conclusions:

  • Quantitative neuroproteomics is a powerful tool for understanding brain molecular mechanisms.
  • Standardized protocols are needed to advance the field of neuroproteomics.
  • This review provides a foundation for researchers in neuroproteomics.