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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Updated: Jul 4, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Production systems for recombinant antibodies.

Thomas Schirrmann¹, Laila Al-Halabi, Stefan Dübel

¹Technische Universität Braunschweig, Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Spielmannstr. 7., 38106 Braunschweig, Germany.

Frontiers in Bioscience : a Journal and Virtual Library

|May 30, 2008

Summary

Recombinant antibody production systems are reviewed, covering bacteria, yeast, insects, plants, and animals. Novel strategies address glycosylation and Fc-independent therapies for diverse applications.

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Area of Science:

Biotechnology
Protein Engineering

Background:

Recombinant antibodies are a rapidly expanding class of therapeutic proteins and critical diagnostic reagents.
Increasing demand necessitates diverse production systems, including bacteria, yeast, fungi, insect cells, mammalian cells, plants, and animals.

Purpose of the Study:

To review current recombinant antibody production systems.
To analyze the advantages and limitations of each system for specific applications.

Main Methods:

Review of existing literature on recombinant antibody production platforms.
Comparative analysis of different expression systems (bacteria, yeast, insect, mammalian, plant, animal).

Main Results:

Mammalian cell lines are currently preferred for therapeutic antibody production due to glycosylation concerns.
Emerging strategies enable human-like glycosylation in non-mammalian systems (yeast, insects, plants).
Fc-independent therapeutic strategies (bispecific antibodies, scFv fusion proteins) can be produced in bacteria.

Conclusions:

A variety of recombinant antibody production systems exist, each with unique strengths and weaknesses.
Technological advancements are overcoming limitations like glycosylation, expanding production possibilities.
The choice of production system depends on the intended application of the recombinant antibody.