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Related Experiment Video

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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

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The papillomavirus E2 DNA binding domain.

Gonzalo de Prat-Gay¹, Kevin Gaston, Daniel O Cicero

¹Instituto Leloir and IIBBA-CONICET, Av. Patricias Argentinas 435, (1405) Buenos Aires, Argentina. gpratgay@leloir.org.ar

Frontiers in Bioscience : a Journal and Virtual Library

|May 30, 2008

Summary

The E2 DNA binding domain of human papillomavirus strain 16 is crucial for viral activity. Understanding its structure, dynamics, and binding mechanisms offers new avenues for antiviral drug development.

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Area of Science:

Molecular Biology
Virology
Structural Biology

Background:

The E2 protein is a master regulator in papillomaviruses, with its DNA binding domain (E2 DBD) mediating essential viral functions.
Human papillomavirus (HPV) is a significant human pathogen, and its E2 protein plays a critical role in viral replication and pathogenesis.

Purpose of the Study:

To review the solution properties of the E2 DNA binding domain from human papillomavirus strain 16.
To integrate structural, dynamic, folding, stability, and DNA binding data.
To explore the implications for antiviral development and fundamental DNA recognition mechanisms.

Main Methods:

Review of existing literature integrating structural, biophysical, and biochemical data.
Analysis of solution properties including dynamics, folding, and stability.
Examination of DNA binding mechanisms and conformational equilibria.

Main Results:

The E2 DNA binding domain exhibits complex solution properties, including specific folding and conformational dynamics.
Its DNA binding mechanism is intricate, involving conformational changes and equilibria.
The unique fold of the E2 DBD, shared with Epstein-Barr nuclear antigen 1 (EBNA1), suggests functional parallels among viral origin-binding proteins.

Conclusions:

Understanding the E2 DNA binding domain's properties is key to deciphering papillomavirus biology.
These insights provide a foundation for developing novel antiviral strategies targeting HPV.
The E2 DBD serves as a valuable model for fundamental studies in DNA recognition and protein folding.