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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
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Chromatin remodeling and SWI/SNF2 factors in human disease

Juraj Kokavec1, Jarmila Podskocova, Jiri Zavadil

  • 1Pathologic Physiology and Center for Experimental Hematology, Charles University in Prague, First Faculty of Medicine, U nemocnice 5, Prague 12853, Czech Republic.

Frontiers in Bioscience : a Journal and Virtual Library
|May 30, 2008
PubMed

Insights

Altering SWI/SNF2 chromatin remodelers disrupts organismal balance, causing human diseases. Understanding these ATPases

Area of Science:

  • Molecular biology
  • Genetics
  • Human pathology

Background:

  • Chromatin structure is crucial for development and homeostasis.
  • SWI/SNF2 superfamily remodelers use ATP hydrolysis to alter DNA-protein interactions.
  • Dysregulation of these factors causes human diseases.

Purpose of the Study:

  • To provide a translational overview of human pathologic physiology related to the SWI/SNF2 superfamily.
  • To integrate recent basic and clinical research findings.
  • To elucidate the mechanistic consequences of SWI/SNF2 ATPase dose alterations.

Main Methods:

  • Review of existing literature.
  • Synthesis of basic science findings.
  • Analysis of clinical research data.

Main Results:

  • Loss-of-function or increased activity of SWI/SNF2 factors leads to distinct human pathologies.
  • Mechanistic insights into the consequences of altered SWI/SNF2 ATPase levels are discussed.

Conclusions:

  • The SWI/SNF2 superfamily plays a critical role in maintaining cellular and organismal health.
  • Altered dosage of these chromatin remodelers has significant implications for human disease.
  • Further human subject-based studies are essential for a comprehensive understanding.

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