Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis

The group A rotavirus strain 69M has recently been classified as a new rotavirus serotype, G8, based on the antigenic specificity of its VP7 outer capsid glycoprotein. The fourth gene of 69M, which encodes the other outer capsid protein VP4, was sequenced. The gene is 2362 nucleotides in length and contains one long open reading frame beginning at the 10th nucleotide from the 5' end and terminating with a stop codon 22 bases from the 3' end. The encoded VP4 contains 776 amino acids. Comparative analysis of the deduced amino acid sequence with other rotavirus strains demonstrated that the VP4 of strain 69M shares 68.9 to 74.5% amino acid identity with the VP4 from strains known to represent four human rotavirus VP4 genetic alleles and 74.9 to 86.2% identity with VP4 from various animal rotaviruses. Northern blot hybridization with either radiolabeled ssRNA transcripts from 69M or cloned 69M gene 4 cDNA as probes indicated that the fourth gene of strain 69M is genetically distinct from any group A rotavirus gene 4 described thus far. These data suggest that at least one additional antigenic specificity of VP4 exists in circulating human group A rotavirus strains.