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Updated: Jul 4, 2026

In Ovo Electroporations of HH Stage 10 Chicken Embryos

In Ovo Electroporations of HH Stage 10 Chicken Embryos

Published on: November 1, 2007

Method for electroporation for the early chick embryo.

Jun Hatakeyama¹, Kenji Shimamura

¹Division of Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.

Development, Growth & Differentiation

|May 31, 2008

Summary

This study presents a novel electroporation method for chick embryo culture. This technique allows for precise control over gene misexpression during early embryonic development.

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Area of Science:

Developmental biology
Molecular biology
Embryology

Background:

In vitro whole-embryo culture is crucial for studying early embryogenesis.
New's culture method is a widely adopted technique for chick embryo research.

Purpose of the Study:

To describe a new electroporation method for chick embryos.
To enable temporally and spatially controlled gene misexpression in gastrulating embryos.

Main Methods:

Utilizing New's in vitro whole-embryo culture system.
Applying electroporation for exogenous gene delivery.
Detailed procedures and device information provided.

Main Results:

Successful misexpression of exogenous genes achieved.

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Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Published on: May 2, 2010

Related Experiment Videos

Last Updated: Jul 4, 2026

In Ovo Electroporations of HH Stage 10 Chicken Embryos

In Ovo Electroporations of HH Stage 10 Chicken Embryos

Published on: November 1, 2007

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Published on: May 2, 2010

Demonstration of temporally and spatially controlled gene manipulation.

Experimental examples validating the method.

Conclusions:

The described electroporation method enhances the utility of chick whole-embryo culture.
This technique offers precise genetic manipulation for studying early embryogenesis.