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In Ovo Electroporations of HH Stage 10 Chicken Embryos
Published on: November 1, 2007
Method for electroporation for the early chick embryo
Jun Hatakeyama1, Kenji Shimamura
1Division of Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
Development, Growth & Differentiation
|May 31, 2008
Abstract:
In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures, and some experimental examples are presented.
