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Published on: September 12, 2011
Electroporation as an efficient method of gene transfer
Noritaka Odani1, Kodai Ito, Harukazu Nakamura
1Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan.
Abstract:
Gene transfer by electroporation is an indispensable method for the study of developmental biology, especially for the study using chick embryos. Here we briefly review the principles of the method, and its application to chick embryos. Methods of transient misexpression and long-term misexpression by retrovirus vector or transposon system, and knockdown by small interference RNA are reviewed.
