Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recombinant human thyroid-stimulating hormone: initial bioactivity assessment using human fetal thyroid cells.

G K Huber1, P Fong, E S Concepcion

  • 1Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029.

The Journal of Clinical Endocrinology and Metabolism
|June 1, 1991
PubMed
Summary

Recombinant human thyroid-stimulating hormone (rec-hTSH) is bioactive and stimulates thyroid cells. This potent glycoprotein hormone shows potential as a diagnostic and therapeutic reagent.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review lettersยท2026
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review lettersยท2025
Same author

Role of probiotics in chronic rhinosinusitis: a systematic review of randomised, controlled trials.

The Journal of laryngology and otologyยท2023
Same author

Open space, not reduced herbivory, facilitates invasion of a marine macroalga, implying it is a disturbance-mediated "passenger" of change.

Marine environmental researchยท2021
Same author

Protective effect of oral contraceptive against <i>Helicobacter pylori</i> infection in US adult females: NHANES 1999-2000.

Epidemiology and infectionยท2021
Same author

A tale of two algal blooms: Negative and predictable effects of two common bloom-forming macroalgae on seagrass and epiphytes.

Marine environmental researchยท2018

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Thyroid-stimulating hormone (TSH) is crucial for thyroid function.
  • Recombinant human TSH (rec-hTSH) is a new preparation for potential clinical use.

Purpose of the Study:

  • To assess the bioactivity of rec-hTSH.
  • To evaluate its potential for clinical applications.

Main Methods:

  • Human fetal thyroid cells were used to test rec-hTSH.
  • Measurements included cyclic adenosine monophosphate (cAMP) release and human thyroglobulin (hTg) secretion.
  • Thyroid cell growth and doubling time were assessed.

Main Results:

  • rec-hTSH demonstrated dose-dependent increases in cAMP release and hTg secretion.

Related Experiment Videos

  • Biopotency was estimated at 5.6 IU/mg, with a bioimmune ratio of 0.55.
  • rec-hTSH significantly reduced thyroid cell doubling time, indicating growth stimulation.

    • Conclusions:

    • rec-hTSH is a potent preparation in a human thyroid cell system.
    • It exhibits significant bioactivity and stimulates thyroid cell growth.
    • rec-hTSH holds potential as a future International Standard and a diagnostic/therapeutic reagent.