Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FINDER converts zero-background kinetic fingerprinting into area-scalable attomolar biomarker detection.

bioRxiv : the preprint server for biology·2026
Same author

ASPL-driven subunit exchange remodels VCP/p97 hexamers and is impaired by a multisystem proteinopathy mutation.

bioRxiv : the preprint server for biology·2026
Same author

Npl4 decodes polyubiquitin length and gates D1-D2 coupling in human VCP/p97.

bioRxiv : the preprint server for biology·2026
Same author

Localized ribosome access and distal tuning via the <i>Listeria prfA</i> RNA thermometer.

bioRxiv : the preprint server for biology·2026
Same author

Single-molecule microscopy of biomolecular condensates: From liquid droplets to heterogeneous networks.

Current opinion in structural biology·2026
Same author

A leader-repeat hairpin blocks extraneous CRISPR RNA production in diverse CRISPR-Cas13 systems.

The EMBO journal·2026

Related Experiment Video

Updated: Jul 4, 2026

Single-Molecule F&ouml;rster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

Do-it-yourself guide: how to use the modern single-molecule toolkit.

Nils G Walter1, Cheng-Yen Huang, Anthony J Manzo

  • 1Department of Chemistry, Single Molecule Analysis Group, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, USA. nwalter@umich.edu

Nature Methods
|May 31, 2008
PubMed
Summary

Single-molecule microscopy offers ultimate sensitivity for studying biological systems, from molecules to cells. This review covers key techniques and guides their application in diverse biosciences fields.

More Related Videos

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

Related Experiment Videos

Last Updated: Jul 4, 2026

Single-Molecule F&ouml;rster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Single-molecule microscopy provides unprecedented sensitivity for biological research.
  • It enables the study of systems ranging from small molecules to living cells.
  • This field holds revolutionary potential for modern biosciences.

Purpose of the Study:

  • To survey the current state-of-the-art in single-molecule tools.
  • To provide guidelines for selecting appropriate single-molecule techniques.
  • To highlight applications across various scientific disciplines.

Main Methods:

  • Fluorescence spectroscopy
  • Tethered particle microscopy
  • Optical and magnetic tweezers
  • Atomic force microscopy

Main Results:

  • Comprehensive overview of advanced single-molecule microscopy techniques.
  • Guidelines for technique selection based on specific research needs.
  • Demonstration of broad applicability in structural biology, enzymology, nanotechnology, and systems biology.

Conclusions:

  • Single-molecule microscopy is a powerful and versatile toolkit.
  • The choice of technique depends on the specific biological question and system.
  • This field continues to advance and impact multiple areas of bioscience research.