Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptome signature for host directed antiviral reprogramming by Nimba & Triphala in macrophage Dengue virus infection models.

Scientific reports·2026
Same author

Human RNA polymerase III termination favors decomposition over facilitated recycling.

Nature communications·2026
Same author

Design, development and performance evaluation of a portable whole-body monitor for in-vivo monitoring of high energy photon emitters.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2026
Same author

Ultrafast and specific miRNA quantification via single-molecule fluorescence quenching kinetics.

Communications biology·2026
Same author

A Unifying Mechanism for Shared Splicing Aberrations in Splicing Factor Mutant Cancers.

bioRxiv : the preprint server for biology·2025
Same author

Adherence to Actigraphic Devices in Elementary School-Aged Children: Systematic Review and Meta-Analysis.

Journal of medical Internet research·2025
Same journal

Efficient evidence-based genome annotation with EviAnn.

Nature methods·2026
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
See all related articles

Related Experiment Video

Updated: Jul 4, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

A practical guide to single-molecule FRET.

Rahul Roy1, Sungchul Hohng, Taekjip Ha

  • 1Department of Physics, University of Illinois at Urbana-Champaign, 1110 West Green Street, Urbana, Illinois 61801, USA.

Nature Methods
|May 31, 2008
PubMed
Summary
This summary is machine-generated.

This guide introduces single-molecule fluorescence resonance energy transfer (smFRET) for biologists. It details experimental design, sample prep, and data analysis for studying molecular dynamics, making smFRET more accessible.

More Related Videos

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Related Experiment Videos

Last Updated: Jul 4, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Area of Science:

  • Biophysics
  • Biochemistry
  • Molecular Biology

Background:

  • Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful technique for studying molecular dynamics.
  • Its application has grown significantly in biology, but it remains largely practiced by biophysicists.
  • Biologists often face challenges in adopting smFRET for their research.

Purpose of the Study:

  • To provide a practical guide for biologists on utilizing smFRET.
  • To enable the study of immobilized molecules and reaction trajectories.
  • To facilitate the broader application of smFRET in biological research.

Main Methods:

  • Focus on immobilized molecule studies for long-timescale measurements (1 ms to minutes).
  • Detailed discussion of experimental design, sample preparation, and single-molecule detection.
  • Guidance on data analysis specific to smFRET experiments.

Main Results:

  • Demonstrates how to build a cost-effective smFRET instrument using off-the-shelf components.
  • Provides reliable protocols and freely available software for operation.
  • Empowers biologists to conduct their own smFRET experiments.

Conclusions:

  • smFRET can be made more accessible to biologists with practical guidance.
  • The technique allows detailed investigation of single-molecule reaction dynamics.
  • This guide aims to lower the barrier to entry for smFRET in biological research.