The spinotrigeminal pathway and its spatial relationship to the origin of trigeminospinal projections in the rat

The anterograde transport of horseradish peroxidase and tritiated amino acids was used to examine the distribution and morphology of spinal afferent fibers terminating in the rat spinal trigeminal complex. The results confirm the existence of a direct, ipsilateral projection from the spinal cord which is distributed exclusively to the deepest layers of the medullary dorsal horn narrow regions subjacent to the spinal trigeminal tract in trigeminal nucleus interpolaris, trigeminal nucleus oralis and the trigeminal main sensory nucleus. Spinal inputs also terminated in the insular trigeminal-cuneatus lateralis nucleus which is a distinct component of the interstitial system of the spinal trigeminal tract. The spinal afferent fibers which terminated in the dorsolateral parts of the spinal trigeminal complex arose from the dorsal column funiculi, while those that terminated in ventral parts of the complex arose from both the dorsal column and lateral funiculi. The tritiated amino acid experiments indicate that at least part of the spinotrigeminal pathway originates from cells located in the cervical spinal dorsal horn. The present findings also document a complex spatial relationship between the spinotrigeminal and trigeminospinal pathways which includes an extensive overlap between spinotrigeminal fibers and spinal projecting neurons in each of the lateralmost regions of the complex. This spatial overlap supports the existence of anatomical substrates which may underlie functional reciprocal loops between the spinal trigeminal complex and cervical spinal cord. Since these regions are primarily concerned with the processing of sensory information from lateral and posterior parts of the face, it follows that the spinotrigeminal pathway may be primarily concerned with the integration of head and neck functions. In addition, the spatial convergence of spinal inputs and the distribution of other trigeminal efferent neurons suggests that part of the spinotrigeminal pathway may be involved in spino-trigemino-thalamic and spino-trigemino-cerebellar pathways in parallel with other spinobulbar pathways in the medulla.