Related Experiment Video
Updated: Jul 4, 2026
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
Imported PER-1 producing Pseudomonas aeruginosa, PER-1 producing Acinetobacter baumanii and VIM-2-producing
Dora Szabó1, Julia Szentandrássy, Zsuzsa Juhász
1Institute of Medical Microbiology, Semmelweis University, Budapest, H-1089, Nagyvárad tér 4., Hungary. szabdor@net.sote.hu
Introduction:
Pseudomonas aeruginosa and Acinetobacter baumanii are important nosocomial pathogens with wide intrinsic resistance. However, due to the dissemination of the acquired resistance mechanisms, such as extended-spectrum beta-lactamase (ESBL) and metallo beta-lactamase (MBL) production, multidrug resistant strains have been isolated more often.
Case Presentation:
We report a case of a Hungarian tourist, who was initially hospitalized in Egypt and later transferred to Hungary. On the day of admission PER-1-producing P. aeruginosa, PER-1 producing A. baumannii, SHV-5-producing Klebsiella pneumoniae and VIM-2-producing P. aeruginosa isolates were subcultured from the patient's samples in Hungary. Comparing the pulsed-field gel electrophoresis (PFGE) patterns of the P. aeruginosa strains from the patient to the P. aeruginosa strains occurring in this hospital, we can state that the PER-1-producing P. aeruginosa and VIM-2-producing P. aeruginosa had external origin.
Conclusion:
This is the first report of PER-1-producing P. aeruginosa,and PER-1-producing A. baumanii strains in Hungary. This case highlights the importance of spreading of the beta-lactamase-mediated resistance mechanisms between countries and continents, showing the importance of careful screening and the isolation of patients arriving from a different country.
Insights
Multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii strains producing carbapenemases were identified in Hungary. These resistant bacterial strains highlight the global spread of antimicrobial resistance and the need for patient screening.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Pseudomonas aeruginosa and Acinetobacter baumannii are significant nosocomial pathogens.
- Increasing prevalence of multidrug-resistant strains due to acquired resistance mechanisms like extended-spectrum beta-lactamases (ESBLs) and metallo beta-lactamases (MBLs).
Observation:
- A case of a Hungarian tourist hospitalized in Egypt and later transferred to Hungary.
- Isolation of PER-1-producing P. aeruginosa, PER-1-producing A. baumannii, SHV-5-producing Klebsiella pneumoniae, and VIM-2-producing P. aeruginosa from patient samples.
- Pulsed-field gel electrophoresis (PFGE) indicated that PER-1 and VIM-2 producing P. aeruginosa strains were of external origin.
Findings:
- First report of PER-1-producing P. aeruginosa and PER-1-producing A. baumannii in Hungary.
- Identification of multidrug-resistant strains with acquired resistance mechanisms in a traveler.
Implications:
- Highlights the international spread of beta-lactamase-mediated resistance mechanisms.
- Emphasizes the importance of rigorous screening and isolation protocols for patients arriving from different countries.
- Underscores the global challenge posed by multidrug-resistant organisms (MDROs) in healthcare settings.
