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Effects of olanzapine on glucose transport, proliferation and survival in C2C12 myoblasts

Giovanni Tulipano1, PierFranco Spano, Daniela Cocchi

  • 1Division of Pharmacology and Toxicology, Department of Biomedical Sciences and Biotechnologies, University of Brescia, Brescia, Italy. tulipano@med.unibs.it

Molecular and Cellular Endocrinology
|June 3, 2008
PubMed

Insights

Atypical antipsychotics like olanzapine directly impact muscle cell glucose uptake, potentially influencing diabetes risk. Clozapine showed no effect on glucose transport in this study.

Area of Science:

  • Pharmacology
  • Cell Biology
  • Metabolism

Background:

  • Atypical antipsychotics are associated with increased diabetes risk.
  • The direct cellular mechanisms underlying this risk are not fully understood.

Purpose of the Study:

  • To investigate the direct effects of atypical antipsychotics on skeletal muscle cell functions.
  • To determine the potential diabetic liability of these drugs at the cellular level.

Main Methods:

  • In vitro study using mouse skeletal muscle satellite cells (C2C12).
  • Assessed basal and insulin-stimulated glucose uptake (2-deoxyglucose uptake).
  • Evaluated effects on cell proliferation, survival, differentiation, and myotube growth.

Main Results:

  • Olanzapine and alpha-methyl-5-HT dose-dependently stimulated glucose uptake in C2C12 myoblasts.
  • Clozapine did not affect glucose transport.
  • Olanzapine increased plasma membrane GLUT4 abundance, suggesting enhanced glucose transport.
  • Neither clozapine nor olanzapine affected myoblast/myotube proliferation, survival, or growth.

Conclusions:

  • Olanzapine enhances glucose uptake in muscle cells, potentially through GLUT4 translocation.
  • These findings provide cellular insights into the metabolic side effects of atypical antipsychotics.
  • The observed in vitro effects align with in vivo observations of glucose changes prior to weight gain.

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