A simple method for AFM tip characterization by polystyrene spheres
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Updated: Jul 4, 2026

Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
Published on: March 16, 2020
Zhi-Gang Zeng1, Guo-dong Zhu, Zhang Guo
1Department of Materials Science, Fudan University, 220 Handan Road, Shanghai 200433, China.
Abstract:
An AFM image would not be the true topography of a surface because of the limitation of a finite size of the tip. The true topography of the surface can be deduced if we can know the tip shape. In this paper a simple method has been established to determine the profile of an AFM tip. A geometrical model for the tip and a spherical object has been proposed to show the procedure for deducing the tip shape from AFM images. Isolated spheres and closely packed spheres with different diameters have been observed to confirm the tip shape by this method. It is a non-destructive method to determine the tip shape and the results can be used for future reconstruction of an AFM image.

