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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Published on: February 6, 2018
Study of inter- and intra-individual variation of meiotic segregation patterns in t(3;15)(q27;q13) boars
K Massip1, H Berland, N Bonnet
1UMR 444 INRA-ENVT Génétique Cellulaire, Ecole Nationale Vétérinaire de Toulouse, BP 87614, 31076 Toulouse Cedex 3, France.
Abstract:
Constitutional chromosomal rearrangements are relatively frequent genetic anomalies in both man and pigs. Among them, reciprocal translocations, present a specific meiotic segregation pattern. The potential "individual" effect of the t(3;15)(q27,q13) translocation was studied using SpermFISH to analyze the meiotic segregation patterns of three boars carrying this rearrangement. Three samples were taken at different times from one of these boars to analyze a potential "time" effect. No "time" or "individual" effect was found in this study. These results should allow more efficient management of certain reciprocal translocations in pig populations but need to be completed by the study of other kinds of chromosomal rearrangements.
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