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Updated: Jul 4, 2026

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
Published on: April 29, 2015
Web-based design and evaluation of T-cell vaccine candidates
James Thurmond1, Hyejin Yoon, Carla Kuiken
1Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Unlabelled:
We present a suite of on-line tools to design candidate vaccine proteins, and to assess antigen potential, using coverage of k-mers (as proxies for potential T-cell epitopes) as a metric. The vaccine design tool uses the recently published 'mosaic' method to generate protein sequences optimized for coverage of high-frequency k-mers; the coverage-assessment tools facilitate coverage comparisons for any potential antigens. To demonstrate these tools, we designed mosaic protein sets for B-clade HIV-1 Gag, Pol and Nef, and compared them to antigens used in a recent human vaccine trial.
Availability:
http://hiv.lanl.gov/content/sequence/MOSAIC/.
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