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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
Published on: March 17, 2023
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Generation of adipose tissue-specific transgenic mouse models
1Department of Anatomy and Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2008
Summary
Researchers developed a new method to create genetically modified mice with specific gene expression in adipose tissue. This technique enables the study of how fat tissue influences metabolism and other bodily functions.
Area of Science:
- Metabolic research
- Genetics
- Endocrinology
Background:
- Adipose tissue is crucial for energy balance, regulating metabolism through triglyceride storage and adipokine secretion.
- Adipose tissue influences feeding, thermogenesis, immunity, and neuroendocrine functions via secreted factors.
- Understanding adipose tissue-specific gene expression is vital for metabolic and endocrine research.
Purpose of the Study:
- To establish a method for creating adipose tissue-specific transgenic mouse models.
- To investigate the role of adipose tissue in energy homeostasis and related functions.
- To enable future studies on the impact of specific gene alterations in fat tissue.
Main Methods:
- Development of a transgenic mouse model.
- Utilizing cholesteryl ester transfer protein (CETP) for adipose tissue-specific gene expression.
- Validation of the transgenic model for research applications.
Main Results:
- Successful preparation of adipose tissue-specific CETP transgenic mice.
- Demonstration of a feasible approach for generating such models.
- Establishment of a platform for studying adipose tissue function.
Conclusions:
- The developed method provides a valuable tool for adipose tissue research.
- This technique can be adapted to create various adipose tissue-specific transgenic models.
- Further research using these models will enhance understanding of metabolic regulation.
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