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Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Related Experiment Video

Updated: Jul 4, 2026

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
09:20

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

Differentiation of adipose stem cells.

Bruce A Bunnell1, Bradley T Estes, Farshid Guilak

  • 1Department of Pharmacology, Center for Gene Therapy, Division of Gene Therapy, Tulane National Primate Research Center, Covington, LA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2008
PubMed
Summary

Adipose stem cells (ASCs) from adult tissues self-renew and differentiate into multiple cell types. This chapter details ASC background and multilineage differentiation potential.

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Last Updated: Jul 4, 2026

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
09:20

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells
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Published on: March 5, 2019

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
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Published on: February 9, 2022

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Tissue Engineering

Background:

  • Stem cells are defined by self-renewal and differentiation capabilities.
  • Adult tissues, including adipose tissue, harbor stem cell populations.
  • Adipose stem cells (ASCs) share similarities with bone marrow-derived mesenchymal stem cells.

Purpose of the Study:

  • To provide background on adipose stem cells.
  • To detail the multilineage differentiation of ASCs.
  • To explore osteogenic, adipogenic, chondrogenic, and neurogenic differentiation pathways.

Main Methods:

  • Isolation and characterization of adipose stem cells.
  • In vitro culture and induction of differentiation.
  • Assessment of phenotypic, biochemical, and functional properties post-differentiation.

Main Results:

  • Adipose tissue yields stem cells with high proliferation capacity.
  • ASCs demonstrate multilineage differentiation potential.
  • Differentiated cells acquire specialized cell properties.

Conclusions:

  • Adipose stem cells represent a valuable source for regenerative medicine.
  • Understanding ASC differentiation is crucial for therapeutic applications.
  • ASCs offer a promising alternative to other mesenchymal stem cell sources.