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Updated: Jul 4, 2026

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
Published on: March 6, 2018
Flow cytometry on the stromal-vascular fraction of white adipose tissue
Danett K Brake1, C Wayne Smith
1Section of Leukocyte Biology, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.
Abstract:
Adipose tissue contains cell types other than adipocytes that may contribute to complications linked to obesity. For example, macrophages have been shown to infiltrate adipose tissue in response to a high-fat diet. Isolation of the stromal-vascular fraction of adipose tissue allows one to use flow cytometry to analyze cell surface markers on leukocytes. Here, we present a technical approach to identify subsets of leukocytes that differentially express cell surface markers.
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