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Related Experiment Video

Updated: Jul 4, 2026

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
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Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Published on: July 16, 2019

Decrease in toll-like receptors 2 and 4 in the spleen of mouse with endotoxic tolerance.

B Zhong1, H Ying Ma, Q Yang

  • 1Department of Pathogenic Biology, School of Medicine, Suzhou University, 199 Reng-al Roud, Dushu Lake Higher Eduction Town, Suzhou, Jiangsu 215123, PR China.

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|June 3, 2008
PubMed
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Endotoxin tolerance in mice involves decreased toll-like receptor (TLR) 2 and TLR4 in the spleen. TLR4 is key for tolerance induction, and TLR2 shows cross-tolerance after lipopolysaccharide challenge.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Endotoxin tolerance is characterized by reduced toll-like receptor (TLR) expression.
  • The spleen, a major lymphatic organ, is crucial for immune responses and inflammation.
  • Investigating TLR2 and TLR4 in the spleen is vital for understanding endotoxin tolerance.

Purpose of the Study:

  • To investigate the expression and localization of toll-like receptor (TLR) 2 and TLR4 proteins in the spleen of endotoxin-tolerant mice.
  • To determine the role of TLR4 in endotoxin tolerance induction.
  • To examine the cross-tolerance of TLR2 following lipopolysaccharide (LPS) challenge in tolerant mice.

Main Methods:

  • Quantitative Western blotting to detect TLR2 and TLR4 protein levels.
  • Immunohistochemistry to visualize TLR2 and TLR4 protein localization in the spleen.

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Last Updated: Jul 4, 2026

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
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Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
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  • Real-time quantitative PCR to analyze IL1-beta mRNA expression in the spleen.

    • Main Results:

    • Mice pretreated with LPS (0.5 mg/kg/day for 14 days) showed decreased TLR2 and TLR4, developing endotoxin tolerance.
    • In tolerant mice, a subsequent LPS bolus (10 mg/kg) did not alter TLR2 or TLR4 protein levels.
    • IL1-beta mRNA expression remained unchanged in the spleen of endotoxin-tolerant mice after LPS challenge.

    Conclusions:

    • Toll-like receptor 4 (TLR4) is identified as a primary target in the induction of endotoxin tolerance.
    • Cross-tolerance of TLR2 is demonstrated in vivo following lipopolysaccharide challenge in endotoxin-tolerant mice.