Related Experiment Video
Updated: Jul 4, 2026
Establishing a Device for Sleep Deprivation in Mice
Published on: September 22, 2023
Sleep is increased by weight gain and decreased by weight loss in mice
Zhiwei Guan1, Alexandros N Vgontzas, Edward O Bixler
1Department of Psychiatry, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Objective:
To determine whether weight loss could reverse excessive sleep in high-fat diet-induced obesity.
Design:
Three groups of mice participated in the study. A weight gain/loss group was fed with high-fat food for 6 weeks (weight gain), and regular food again for 4 weeks (weight loss). A control group and a weight gain only group were fed with regular food and high-fat food, respectively, for 10 weeks after the baseline.
Participants:
Adult male C57BL/6 mice.
Measurements:
The amounts of wake, rapid eye movement sleep (REMS) and non-REM sleep (NREMS) were determined at week 0 (baseline), week 6, and week 10.
Results:
The weight gain/loss group displayed a significant decrease in wakefulness and increases in NREMS and episodes of NREMS during 6 weeks of weight gain, which were reversed during subsequent 4 weeks of weight loss. The weight gain only group displayed significant decrease in wakefulness and increase of NREMS and REMS at both week 6 and week 10. The control group did not show significant sleep alterations during the experiment.
Conclusion:
These observations indicate that sleep alterations induced by weight gain are reversed by weight loss in obese animals. These data may shed light on the mechanisms underlying the well-established association between obesity and sleepiness in humans and may lead to new therapeutic strategies for these 2 increasingly prevalent problems in the modern societies.
