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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Related Experiment Video

Updated: Jul 4, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

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Published on: May 27, 2008

Quantitative phase imaging with a scanning transmission x-ray microscope.

M D de Jonge1, B Hornberger, C Holzner

  • 1Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, USA. martin.dejonge@synchrotron.org.au

Physical Review Letters
|June 4, 2008
PubMed
Summary
This summary is machine-generated.

Researchers reconstructed quantitative phase using differential phase contrast imaging with scanning transmission x-ray microscopy and 2.5 keV x-rays. This study presents the technique’s theory, measurements, and interpretation.

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Area of Science:

  • X-ray microscopy
  • Phase contrast imaging
  • Quantitative phase reconstruction

Background:

  • Differential phase contrast (DPC) imaging is a powerful technique for visualizing transparent specimens.
  • Scanning transmission x-ray microscopy (STXM) offers high spatial resolution for materials analysis.

Purpose of the Study:

  • To present a method for quantitative phase reconstruction using DPC images obtained from STXM.
  • To demonstrate the application of this technique with 2.5 keV x-rays.

Main Methods:

  • Acquisition of differential phase contrast images using a scanning transmission x-ray microscope.
  • Application of quantitative phase reconstruction algorithms to the DPC data.
  • Utilizing 2.5 keV x-rays for imaging.

Main Results:

  • Successful quantitative phase maps were generated from DPC images.
  • The theoretical framework underpinning the phase reconstruction was detailed.
  • Experimental measurements and their interpretations were provided.

Conclusions:

  • Quantitative phase reconstruction is feasible with STXM and DPC imaging.
  • The presented method provides a valuable tool for nanoscale phase imaging.
  • This technique enables detailed characterization of materials based on their phase information.