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Updated: Jul 4, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B
Xiaoyun Wu1, You Zhou, Ke Zhang
1State Key Laboratory of Virology, the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China.
Abstract:
Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunoprecipitation and confocal immunofluorescence analysis in cells with transient expression of NS5B and M2PK as well as in a HCV replicon-bearing cell line. Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells.
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