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Updated: Jul 4, 2026
09:58
Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
Published on: June 27, 2020
The E2F3-Oncomir-1 axis is activated in Wilms' tumor.
Eric J Kort1, Leslie Farber, Maria Tretiakova
1Laboratory of Molecular Epidemiology, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
Cancer Research
|June 4, 2008
Summary
The E2F3-Oncomir-1 pathway is activated in Wilms
Area of Science:
- Genomics
- Molecular Biology
- Oncology
Background:
- The microRNA (miRNA) cluster Oncomir-1 is known to be oncogenic and is located on chromosome 13.
- Previous in vitro research indicated that the transcription factor E2F3 regulates Oncomir-1 transcription.
Purpose of the Study:
- To investigate the deregulation of the E2F3-Oncomir-1 axis in primary human tumors.
- To provide the first evidence of this axis's involvement in Wilms' tumor (WT).
Main Methods:
- Combined expression profiling of both mRNA and miRNAs in Wilms' tumor samples.
- Analysis of RNA expression signatures to identify E2F3 gene signatures.
- Immunohistochemistry to validate E2F3 protein expression.
- Quantitative PCR to confirm miRNA expression levels.
Main Results:
- An E2F3 gene signature was activated in all analyzed WT samples, distinguishing them from other kidney tumors.
- E2F3 protein expression was found to be lowest in early-stage tumors and highest in metastatic tissue.
- Members of the Oncomir-1 family were most highly expressed in WT compared to other kidney tumor subtypes.
- Quantitative PCR confirmed overexpression of these miRNAs in WT relative to normal kidney tissue.
Conclusions:
- The E2F3-Oncomir-1 axis is suggested to be activated in Wilms' tumor.
- The study highlights the utility of integrated genomics, combining gene signature analysis with miRNA expression profiling, for identifying perturbed protein-miRNA interactions in diseases.
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