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Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells
Punnee Nusuetrong1, Thitima Pengsuparp, Duangdeun Meksuriyen
1Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Sukhumvit, Bangkok, Thailand.
Abstract:
Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.
Insights
Satratoxin H induces apoptosis in PC12 cells by increasing reactive oxygen species (ROS). Glutathione (GSH) can protect cells, suggesting ROS generation mediates this mycotoxin-induced cell death.
Area of Science:
- Toxicology
- Cell Biology
- Biochemistry
Background:
- Satratoxin H is a mycotoxin known to induce apoptosis in PC12 cells.
- Previous studies suggest a role for p38 MAPK and JNK pathways, modulated by glutathione (GSH).
Purpose of the Study:
- To further elucidate the mechanism of satratoxin H-induced cell death in PC12 cells.
- To investigate the role of reactive oxygen species (ROS) in satratoxin H toxicity.
Main Methods:
- PC12 cells were treated with satratoxin H.
- Apoptosis was assessed via DNA fragmentation and flow cytometry.
- ROS production and lipid peroxidation (malondialdehyde) were measured.
- Effects of GSH incubation were evaluated.
Main Results:
- Satratoxin H induced significant apoptosis in PC12 cells within 24 hours.
- Increased ROS production and lipid peroxidation were observed following satratoxin H exposure.
- Incubation with GSH attenuated the satratoxin H-induced apoptosis and ROS generation.
Conclusions:
- Satratoxin H induces apoptosis in PC12 cells, partly mediated by ROS generation.
- Glutathione (GSH) plays a protective role against satratoxin H toxicity.
- The findings highlight the involvement of oxidative stress in satratoxin H-induced cytotoxicity.
