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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
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RNA editing regulates insect gamma-aminobutyric acid receptor function and insecticide sensitivity

Zeineb Es-Salah1, Bruno Lapied, Gaëlle Le Goff

  • 1IFR QUASAV 149, RCIM Laboratory, UPRES-EA 2647/USC INRA, University of Angers, UFR Sciences, 2 Boulevard Lavoisier, F-49045 Angers Cedex, France.

Neuroreport
|June 4, 2008
PubMed

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...