Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reflections on the 2024 KTA Guideline and the Role of Radioiodine Therapy in Differentiated Thyroid Cancer.

Nuclear medicine and molecular imaging·2025
Same author

Radiation-induced exosomal miR-21 enhances tumor proliferation and invasiveness in breast cancer: implications for poor prognosis in radiotherapy patients.

Experimental hematology & oncology·2024
Same author

Evaluating 64 Cu-DOTA-rituximab as a PET agent in patients with B-cell lymphoma: a head-to-head comparison with 18 F-fluorodeoxyglucose PET/computed tomography.

Nuclear medicine communications·2024
Same author

Selenium levels and their association with thyroid autoimmunity and severe preeclampsia in pregnancy: Insights from a prospective ideal breast milk cohort study.

European thyroid journal·2024
Same author

Feasibility Study of Single-Photon Emission Computed Tomography with Iodine-123 Labeled Metaiodobenzylguanidine for Preclinical Evaluation of Labetalol as a β-Adrenergic Receptor Blocker.

Molecular pharmaceutics·2024
Same author

Overexpression of Both Human Sodium Iodide Symporter (NIS) and BRG1-Bromodomain Synergistically Enhances Radioiodine Sensitivity by Stabilizing p53 through NPM1 Expression.

International journal of molecular sciences·2023

Related Experiment Video

Updated: Jul 4, 2026

Molecular Imaging to Target Transplanted Muscle Progenitor Cells
09:24

Molecular Imaging to Target Transplanted Muscle Progenitor Cells

Published on: March 27, 2013

Molecular-genetic imaging based on reporter gene expression.

Joo Hyun Kang1, June-Key Chung

  • 1Department of Nuclear Medicine, Cancer Research Institute, Tumor Immunity Medical Research Center, College of Medicine, Seoul National University, Seoul, Korea.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
|July 1, 2008
PubMed
Summary

Molecular imaging uses reporter genes for genetic imaging, enabling visualization of biological processes. This technology is advancing cancer diagnosis and therapy through improved drug discovery and stem cell applications.

More Related Videos

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Related Experiment Videos

Last Updated: Jul 4, 2026

Molecular Imaging to Target Transplanted Muscle Progenitor Cells
09:24

Molecular Imaging to Target Transplanted Muscle Progenitor Cells

Published on: March 27, 2013

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
10:04

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Area of Science:

  • Molecular biology
  • Medical imaging
  • Biotechnology

Background:

  • Molecular imaging encompasses proteomic, metabolic, cellular, and genetic processes.
  • Molecular-genetic imaging, a subset, utilizes imaging reporter genes.
  • Reporter genes are crucial for visualizing gene expression and biological phenomena in vivo.

Purpose of the Study:

  • To review the role and applications of molecular-genetic imaging.
  • To highlight the advantages and disadvantages of different imaging modalities.
  • To discuss the potential of reporter gene technology in medicine and drug development.

Main Methods:

  • Overview of three major molecular-genetic imaging modalities: optical (fluorescent, bioluminescent), MRI, and nuclear medicine.
  • Discussion of various reporter genes and probes developed for these modalities.
  • Exploration of reporter gene applications in visualizing gene expression, cellular processes, and tumor mass.

Main Results:

  • Optical imaging offers simplicity and cost-effectiveness, with bioluminescence detecting low gene expression.
  • MRI provides high spatial resolution.
  • Nuclear medicine excels in sensitivity and translation from small-animal studies to clinical practice.

Conclusions:

  • Multimodality imaging reporter genes enhance flexibility and clinical application of reporter gene technologies.
  • Molecular imaging is vital for drug discovery, stem cell therapy, and cancer diagnosis/therapy.
  • Advancements in instrumentation, targets, and probes position molecular-genetic imaging for significant future impact.