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Updated: Jul 4, 2026
10:33
Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
Agromyces terreus sp. nov., isolated from soil
Jung-Hoon Yoon1, Peter Schumann, So-Jung Kang
1Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea. jhyoon@kribb.re.kr
Summary
A novel soil bacterium, Agromyces terreus sp. nov. strain DS-10(T), was identified in Korea. This Gram-positive actinomycete expands the known diversity within the Agromyces genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Soil microbial communities harbor diverse, yet uncharacterized, bacterial species.
- The genus Agromyces comprises Gram-positive actinomycetes with various ecological roles.
- Accurate taxonomic classification is crucial for understanding microbial diversity and function.
Purpose of the Study:
- To taxonomically characterize a novel bacterial strain, DS-10(T), isolated from Korean soil.
- To determine the phylogenetic placement of strain DS-10(T) within the genus Agromyces.
- To propose a new species designation based on polyphasic taxonomic data.
Main Methods:
- Isolation and cultivation of strain DS-10(T) under specific environmental conditions (25°C, pH 7.0-8.0, 0.5-1.0% NaCl).
- Polyphasic taxonomic analysis including cell wall composition (peptidoglycan type B2gamma), whole-cell sugars (galactose, ribose), menaquinones (MK-11, MK-12, MK-10), and major fatty acids (anteiso-C15:0, anteiso-C17:0, iso-C16:0, iso-C15:0).
- Phylogenetic analysis using 16S rRNA gene sequencing and DNA-DNA hybridization to assess relatedness to existing Agromyces species.
Main Results:
- Strain DS-10(T) is a Gram-positive, non-motile, rod-shaped bacterium with specific growth optima and biochemical characteristics.
- 16S rRNA gene sequence analysis placed strain DS-10(T) within the genus Agromyces, with 95.8-99.3% similarity to known species.
- DNA-DNA relatedness and phenotypic data confirmed strain DS-10(T) is distinct from recognized Agromyces species, with a DNA G+C content of 71.1 mol%.
Conclusions:
- Strain DS-10(T) represents a novel species within the genus Agromyces.
- The proposed name for this new species is Agromyces terreus sp. nov.
- The type strain is DS-10(T) (=KCTC 19216(T) =JCM 14581(T)), contributing to the known biodiversity of soil actinomycetes.
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