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Updated: Jul 4, 2026
Viability Assay of Trichoderma stromaticum Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages
Published on: October 20, 2023
Biocontrol potential and polyphasic characterization of novel native Trichoderma strains against Macrophomina
C P Larralde-Corona1, M R Santiago-Mena, A M Sifuentes-Rincón
1Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Blvd Del Maestro esq. Elias Piña, Reynosa, Tamaulipas, Mexico.
Abstract:
Native strains of Trichoderma isolated from sorghum and common bean crop soils were investigated to assess their biocontrol potential over the phytopathogenic fungus Macrophomina phaseolina, isolated from diseased plants. The Trichoderma strains were characterized with a polyphasic approach, which combined the analysis of their morphological characteristics, enzymatic activity, macro- and microculture test results, rDNA restriction patterns (AFLP), ITS1-5.8S-ITS2 rDNA sequences, and protein profiles. The integration of these data sets can be used to select new isolates as biological control agents against native fungal phytopathogens. In general, we observed a positive correlation between the secretion of beta-1,3-glucanase and N-acetylhexosaminidase, and the biocontrol capacities of all the Trichoderma isolates. Strains with the best hyperparasitic behavior against M. phaseolina isolated from diseased bean and sorghum were Trichoderma sp. (TCBG-2) and Trichoderma koningiopsis (TCBG-8), respectively.
