Development of a disposable glucose biosensor using electroless-plated Au/Ni/copper low electrical resistance...

Area of Science:

• Electrochemistry
• Biosensor Technology
• Materials Science

Background:

• Development of efficient glucose biosensors is crucial for diabetes management.
• Existing biosensors often face challenges with electrode resistance and interference.
• Novel electrode materials are needed to improve biosensor performance and manufacturability.

Purpose of the Study:

• To develop and characterize a novel glucose biosensor utilizing a Au/Ni/copper electrode.
• To investigate the low electrical resistance and uniformity of the developed electrode.
• To evaluate the performance of the glucose biosensor for accurate glucose detection.

Main Methods:

• Fabrication of a Au/Ni/copper electrode.
• Immobilization of glucose oxidase (GOD) onto the electrode surface.
• Electrochemical characterization of the biosensor for glucose detection, including cyclic voltammetry and amperometry.

Main Results:

• The Au/Ni/copper electrode exhibited low electrical resistance (< 0.01 Omega) and good uniformity, suitable for mass production.
• The GOD/Au/Ni/copper biosensor detected glucose at a low potential (0.055 V) with high sensitivity (0.85 microA mM(-1)) and a linear range up to 33 mM.
• The biosensor demonstrated a short response time (~3s), excellent reproducibility (R=0.9989), and minimal non-linearity error (3.17%), effectively excluding common interferences.

Conclusions:

• The novel Au/Ni/copper electrode offers significant advantages for glucose biosensor development due to its low resistance and uniformity.
• The developed GOD/Au/Ni/copper biosensor provides sensitive, selective, and reproducible glucose detection at a low operating potential.
• This biosensor technology holds promise for practical applications in glucose monitoring and diagnostics.