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Related Concept Videos

Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

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Related Experiment Video

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Antigenic Liposomes for Generation of Disease-specific Antibodies
10:31

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Published on: October 25, 2018

Exploiting CD38-mediated endocytosis for immunoliposome internalization.

Monia Orciani1, Guido Cavaletti, Vincenzo Fino

  • 1Department of Molecular Pathology and Innovative Therapies, Histology Section, Marche Polytechnic University, Ancona, Italy.

Anti-Cancer Drugs
|June 6, 2008
PubMed
Summary

CD38-targeted immunoliposomes efficiently target cancer cells for drug delivery. This antibody-based therapy leverages CD38 receptor-mediated endocytosis for precise cancer cell suicide, showing great potential in lymphoid tumor treatment.

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Area of Science:

  • Biotechnology
  • Immunology
  • Oncology

Background:

  • CD38 is a cell surface protein upregulated in lymphoid tumors.
  • Antibody interaction with CD38 triggers rapid internalization via endocytosis.
  • This mechanism enables targeted delivery of toxins or drugs to malignant cells.

Purpose of the Study:

  • To prepare and evaluate the functionality of CD38-immunoliposomes for targeted cancer therapy.
  • To investigate the CD38-mediated endocytosis mechanism for drug delivery.

Main Methods:

  • Liposomes were prepared with biotinylated polyethylene glycol-phospholipid and loaded with a fluorescent dye.
  • Anti-CD38 antibody (IB4) was biotinylated and linked to liposomes via streptavidin.
  • CD38+ and CD38- cells were incubated with liposomes and immunoliposomes, analyzed by fluorescence microscopy and cytofluorimetry.

Main Results:

  • CD38+ cells incubated with CD38-immunoliposomes showed significantly higher fluorescence levels.
  • The results confirmed CD38-mediated endocytosis as the specific internalization mechanism.
  • The streptavidin-based coupling strategy did not interfere with cellular functionality.

Conclusions:

  • CD38-immunoliposomes are effectively prepared and demonstrate specific, CD38-mediated cellular uptake.
  • This targeted delivery system shows promise for antibody-based cancer therapy, particularly for lymphoid tumors.
  • The versatile coupling method is adaptable for other antibodies and cell surface markers.