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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

Updated: Jul 4, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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LC-MS for protein characterization: current capabilities and future trends.

Guodong Chen1, Birendra N Pramanik

  • 1Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. guodong.chen@spcorp.com

Expert Review of Proteomics
|June 6, 2008
PubMed
Summary

Liquid chromatography-mass spectrometry (LC-MS) is a powerful tool for protein characterization. This review covers LC-MS strategies for analyzing proteins, including modifications and future directions in proteomics.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • Liquid chromatography-mass spectrometry (LC-MS) has advanced significantly with improved ionization and instrumentation.
  • LC-MS is now a key technology for comprehensive protein analysis.

Purpose of the Study:

  • To review general approaches for protein characterization using LC-MS.
  • To discuss the application of LC-MS in analyzing recombinant proteins and post-translational modifications.
  • To highlight new research directions and future prospects of LC-MS in proteomics.

Main Methods:

  • Bottom-up LC-MS strategies.
  • Top-down LC-MS strategies.
  • Analysis of post-translational modifications (e.g., disulfide bonds, glycosylation, phosphorylation).

Main Results:

  • LC-MS enables detailed characterization of proteins and their modifications.
  • Both bottom-up and top-down approaches are effective for protein analysis.
  • LC-MS is crucial for understanding complex biological systems through proteomics.

Conclusions:

  • LC-MS is an indispensable technology for protein characterization.
  • Future applications of LC-MS in proteomics are promising.
  • Continued advancements in LC-MS will drive new discoveries in biology.