Related Experiment Video
Updated: Jul 4, 2026
OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 20, 2010
[Study on enzyme-assisted extraction of polysaccharides from Dioscorea opposita]
Yuan Zhang1, Qiang Lin, Jing-Na Wei
1Biochemical Engineering College of Beijing Uion University, Beijing 100023, China.
Objective:
To extract polysaccharides from Dioscorea opposita by alpha-amylase and ultrasound.
Method:
The optimum condition of enzyme-assisted extraction has been obtained through orthogonal test according to the yield of polysaccharides.
Result:
A higher yield of polysaccharides was achieved at 55 degrees C, pH 5.5 , with a load of alpha-amylase 10 mg for 1.0 hour, the extraction rate was increased by in compare of sonolysis treatment alone.
Conclusion:
To carry out an enzyme treatment before ultrasound--assisted extraction elevated the yield of polysaccharides.
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