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Hoechst 33258--poly(dG-dC).poly(dG-dC) complexes of three types
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow 119991, Russia. strelcov@eimb.ru
Journal of Biomolecular Structure & Dynamics
|June 7, 2008
Summary
Hoechst 33258, a DNA dye, binds to poly(dG-dC).poly(dG-dC) forming monomeric, dimeric, and tetrameric complexes. These complexes interact with the minor groove of DNA, influencing its biological activity.
Area of Science:
- Molecular Biology
- Biophysics
- Biochemistry
Background:
- Hoechst 33258 is a dsDNA fluorescent dye with known inhibitory effects on DNA-binding proteins and radioprotective properties.
- Its biological activity may stem from interactions with both monomeric and dimeric dsDNA complexes.
Purpose of the Study:
- To investigate the interaction of Hoechst 33258 with the synthetic DNA polymer poly(dG-dC).poly(dG-dC).
- To characterize the types of complexes formed and their spectral properties.
Main Methods:
- UV-vis spectroscopy
- Fluorescent spectroscopy
- Circular dichroism
- Flow-type linear dichroism
Main Results:
- Hoechst 33258 formed three types of complexes with poly(dG-dC).poly(dG-dC): monomeric, dimeric, and tetrameric.
- Spectral properties of these complexes were characterized.
- Monomeric and dimeric complexes competed with distamycin A, a minor groove ligand, for binding.
Conclusions:
- Hoechst 33258 forms multiple types of complexes with poly(dG-dC).poly(dG-dC).
- Both monomeric and dimeric Hoechst 33258 complexes likely bind externally to the minor groove of poly(dG-dC).poly(dG-dC).
