Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flow Cytometry-Based Rapid Assay for Antigen Specific Antibody Relative Affinity in SRBC-Immunized Mouse Models.

International journal of molecular sciences·2025
Same author

Conventional High-Temperature Superconductivity at Ambient Pressure in Zincblende-Like Light-Element Compounds.

Inorganic chemistry·2025
Same author

Pathways to Aromatics in the Catalytic Pyrolysis of a Polyvinylchloride Model Compound Revealed by Operando Photoelectron Photoion Coincidence Spectroscopy.

ChemSusChem·2025
Same author

Autonomous 3D Self-Sensing Hybrid Membrane Actuator for Interactive Communicating.

ACS applied materials & interfaces·2025
Same author

A Lightweight Pig Aggressive Behavior Recognition Model by Effective Integration of Spatio-Temporal Features.

Animals : an open access journal from MDPI·2025
Same author

Enhancing C─C Bond Cleavage of Glycerol Electrooxidation Through Spin-Selective Electron Donation in Pd-PdS<sub>2</sub>-Co<sub>x</sub> Heterostructural Nanosheets.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: Jul 4, 2026

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
19:57

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Published on: March 30, 2014

An efficient method for purification of PCR products for sequencing.

Hao Ma1, Stephen Difazio

  • 1Department of Biology, West Virginia University, Morgantown, WV 26506, USA. hao.ma@mail.wvu.edu

Biotechniques
|June 7, 2008
PubMed
Summary

A novel high-throughput DNA sequencing method uses a simple gel frame to isolate PCR products, yielding high-quality data cost-effectively. This technique simplifies sequencing workflows and is effective for single nucleotide polymorphism discovery.

More Related Videos

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
15:28

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Related Experiment Videos

Last Updated: Jul 4, 2026

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
19:57

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Published on: March 30, 2014

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
15:28

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • High-throughput DNA sequencing is crucial for genetic research.
  • Traditional methods for isolating PCR products can be labor-intensive and prone to contamination.
  • Efficient purification of polymerase chain reaction (PCR) products is essential for reliable sequencing.

Purpose of the Study:

  • To develop a high-throughput, cost-effective DNA sequencing method.
  • To simplify the process of purifying PCR products for sequencing.
  • To enable efficient single nucleotide polymorphism (SNP) discovery.

Main Methods:

  • A novel gel frame system using standard and low-melting point (LMP) agarose was designed.
  • PCR products were isolated using the gel frame and purified by centrifugation.
  • Supernatants containing purified PCR products were directly used in sequencing reactions.

Main Results:

  • The developed method generates high-quality sequencing data.
  • The technique is simple, labor-efficient, and cost-effective.
  • It effectively bypasses issues associated with impure PCR products.

Conclusions:

  • This innovative method offers a streamlined approach to DNA sequencing.
  • It is particularly useful for applications like single nucleotide polymorphism (SNP) discovery in plant genetics.
  • The technique enhances efficiency and reduces costs in high-throughput sequencing workflows.