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Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution

Atze T Das1, Bep Klaver, Mireille Centlivre

  • 1Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, The Netherlands. a.t.das@amc.uva.nl

Retrovirology
|June 7, 2008
PubMed
Summary

Researchers optimized a doxycycline-dependent simian immunodeficiency virus (SIV) variant through in vitro evolution. This enhanced SIV-rtTA replicates efficiently in cells and macaque PBMCs, maintaining doxycycline control for potential vaccine applications.

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