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Updated: Jul 4, 2026

Microfluidics in Assessing Platelet Function

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

Platelet function testing in uraemic patients.

Shir-Jing Ho¹, Rosalie Gemmell, Timothy A Brighton

¹SEALS, Department of Haematology, St George Hospital, Gray St, Kogarah, NSW 2217, Australia. shir-jing.ho@sesiahs.health.nsw.gov.au

Hematology (Amsterdam, Netherlands)

|June 7, 2008

Summary

Platelet dysfunction in chronic renal failure (CRF) causes bleeding. This study found skin bleeding time (SBT) is not reliably predicted by kidney function tests or newer platelet assays.

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Published on: February 14, 2017

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Published on: February 14, 2017

Area of Science:

Nephrology
Hematology
Clinical Pathology

Background:

Chronic renal failure (CRF) is linked to bleeding disorders, primarily due to platelet dysfunction affecting platelet interactions.
The skin bleeding time (SBT) is a known predictor of bleeding in uremic patients, but its accuracy and reproducibility are limited.
Newer rapid platelet function assays exist but haven't been thoroughly evaluated in CRF patients.

Purpose of the Study:

To assess the predictive value of kidney function tests and novel in vitro platelet assays for skin bleeding time (SBT) in patients with renal impairment.
To investigate the correlation between calculated glomerular filtration rate (GFR) and SBT in patients with acute and chronic renal failure.

Main Methods:

A prospective cohort study involving patients with acute and chronic renal impairment.
Analysis of laboratory parameters including GFR, SBT, whole blood platelet aggregation (WBPA), platelet function analyzer (PFA-100), thromboelastograph (TEG), and cone platelet analyzer (CPA).

Main Results:

Calculated GFR showed a weak correlation with SBT (r² = 0.1564), while serum creatinine and urea levels demonstrated even poorer correlation.
The PFA-100 assay was found to be a poor predictor of SBT.
Abnormalities in WBPA and CPA were observed across various SBT ranges, with no clear predictive pattern identified for SBT.

Conclusions:

Serum creatinine and calculated GFR do not reliably predict prolonged SBT in patients with renal impairment.
This pilot study did not identify a definitive in vitro alternative assay to accurately replicate SBT in this patient population.