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Updated: Jul 4, 2026
09:40
Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Aberrant gene expression in deceased transgenic cloned calves
1China Agricultural University, Haidian District, Beijing, PR China. red3stones@vip.sina.com
Animal Reproduction Science
|June 7, 2008
Summary
Transgenic cloning in cattle results in low efficiency and abnormal development. Gene expression analysis revealed widespread decreased expression of development-related genes in aberrant transgenic cloned calves.
Area of Science:
- Animal Biotechnology
- Developmental Biology
- Gene Expression Analysis
Background:
- Transgenic cloning offers potential for livestock improvement but faces challenges.
- Low efficiency and abnormal development are significant issues in cloned animals.
- Aberrant gene expression is suspected to underlie developmental abnormalities in clones.
Purpose of the Study:
- To investigate gene expression patterns in transgenic cloned cattle.
- To identify specific development-related genes affected by transgenic cloning.
- To correlate gene expression changes with abnormal growth and development.
Main Methods:
- Comparative analysis of gene expression in lungs of normal controls and transgenic cloned calves.
- Quantitative assessment of 19 key development-related genes.
- Utilizing established molecular biology techniques for gene expression profiling.
Main Results:
- Transgenic cloned calves exhibited significantly altered gene expression profiles.
- 84.2% of the examined genes showed decreased expression levels.
- A smaller proportion (5.3%) of genes displayed increased expression levels.
Conclusions:
- Transgenic cloning can lead to aberrant gene expression in cattle.
- Altered gene expression is implicated in the abnormal growth and development of transgenic cloned calves.
- This study provides novel insights into the molecular basis of low transgenic cloning efficiency in cattle.
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