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Related Concept Videos

Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Thermosensation01:43

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.

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Related Experiment Video

Updated: Jul 4, 2026

Purification of Endogenous Drosophila Transient Receptor Potential Channels
08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Published on: December 28, 2021

TRP channels entering the structural era.

Rachelle Gaudet1

  • 1Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 01238, USA. gaudet@mcb.harvard.edu

The Journal of Physiology
|June 7, 2008
PubMed
Summary
This summary is machine-generated.

Structural biology advances reveal Transient Receptor Potential (TRP) channel mechanisms. New insights into TRP channel gating and regulation emerge from fragment and whole-channel structural studies.

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Last Updated: Jul 4, 2026

Purification of Endogenous Drosophila Transient Receptor Potential Channels
08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Published on: December 28, 2021

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

Area of Science:

  • Structural biology
  • Molecular physiology

Background:

  • Transient Receptor Potential (TRP) channels are crucial for numerous physiological functions in both neuronal and non-neuronal systems.
  • Understanding the precise molecular mechanisms governing TRP channel gating and regulation is essential for advancing physiological research.

Purpose of the Study:

  • To review recent progress in elucidating TRP channel structure and function using advanced structural biology techniques.
  • To highlight the contributions of different structural biology approaches to understanding TRP channel architecture and gating.

Main Methods:

  • High-resolution structural analysis of TRP channel fragments using a "divide-and-conquer" strategy.
  • Low-resolution imaging of entire TRP channels via electron microscopy to model overall architecture.

Main Results:

  • The "divide-and-conquer" approach provided detailed structural information on TRP channel components.
  • Electron microscopy offered low-resolution models of complete TRP channel structures, enabling architectural analysis.
  • Integration of fragment and whole-channel data provides a foundation for understanding TRP channel gating.

Conclusions:

  • Recent structural biology studies have significantly advanced the understanding of TRP channel gating and regulation.
  • Combining high-resolution fragment data with low-resolution whole-channel structures offers a promising path forward for TRP channel research.
  • Future research will likely focus on integrating these structural insights to build comprehensive models of TRP channel function.