Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human pancreatic ductal cells from non-diabetic donors function as non-professional antigen-presenting cells upon inflammatory cytokine exposure.

Diabetologia·2026
Same author

Protocol to study ductal progenitor-like cells from the adult human pancreas using 3D suspension and methylcellulose-based culture systems.

STAR protocols·2025
Same author

A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans.

Scientific reports·2025
Same author

Characterization of Human Pancreatic Islet Cells Using a Single-Cell Western Blot Platform.

Pancreas·2024
Same author

Trefoil Factor 2 Expressed by the Murine Pancreatic Acinar Cells Is Required for the Development of Islets and for β-Cell Function During Aging.

Diabetes·2024
Same author

Biological hypoxia in pre-transplant human pancreatic islets induces transplant failure in diabetic mice.

Scientific reports·2024

Related Experiment Video

Updated: Jul 4, 2026

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
09:31

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

Published on: June 10, 2016

Minireview: pancreatic progenitor cells--recent studies.

Hsun Teresa Ku1

  • 1Department of Diabetes, Endocrinology, and Metabolism, Beckman Research Institute of City of Hope, Duarte, California 91010, USA. hku@coh.org

Endocrinology
|June 7, 2008
PubMed
Summary

Pancreatic progenitor cells exist in a hierarchy, from rare multipotent progenitors to abundant monopotent ones. This model explains how the pancreas regenerates after injury or during homeostasis.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Gastroenterology

Background:

  • Past pancreatic progenitor cell research relied heavily on histological analyses.
  • Recent studies suggest the existence of diverse pancreatic progenitor cells with varying lineage potentials in developing and adult pancreases.

Purpose of the Study:

  • To propose a hierarchical model for pancreatic progenitor cells.
  • To explain progenitor cell activation based on injury severity and physiological state.
  • To differentiate functional roles of ductal and endocrine progenitor cells.

Main Methods:

  • Review of recent genetic labeling, cell tracing, single-cell analyses, and colony assays.
  • Integration of findings on progenitor cell frequency and lineage potential.

More Related Videos

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
14:39

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

Published on: November 28, 2011

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
09:23

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

Related Experiment Videos

Last Updated: Jul 4, 2026

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
09:31

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

Published on: June 10, 2016

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
14:39

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

Published on: November 28, 2011

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
09:23

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

  • Functional analysis of endocrine progenitor cell activity.

    • Main Results:

    • Pancreatic progenitor cells are proposed to be organized in a hierarchy, with rare, primitive multipotent progenitors at the top and abundant monopotent progenitors at the bottom.
    • This hierarchy explains differential progenitor cell activation in response to varying injury levels and physiological conditions.
    • Ductal cells are functionally heterogeneous, and endocrine progenitor activity is best assessed functionally, not histologically.

    Conclusions:

    • A hierarchical model of pancreatic progenitor cells provides a framework for understanding pancreatic regeneration and homeostasis.
    • Functional assessment is crucial for determining endocrine progenitor cell activity.
    • Future research should focus on the functional heterogeneity of ductal cells and the precise roles of different progenitor populations.